PUBLICATION
Rock2 controls TGFβ signaling and inhibits mesoderm induction in zebrafish embryos
- Authors
- Zhang, Y., Li, X., Qi, J., Wang, J., Liu, X., Zhang, H., Lin, S.C., and Meng, A.
- ID
- ZDB-PUB-090616-47
- Date
- 2009
- Source
- Journal of Cell Science 122(Pt 13): 2197-2207 (Journal)
- Registered Authors
- Li, Xiang, Meng, Anming, Zhang, Haiwen, Zhang, Yu
- Keywords
- Rho kinases, Rock2, TGFβ, Zebrafish, Embryo
- MeSH Terms
-
- Animals
- Body Patterning/physiology
- Cell Line
- Embryonic Induction*
- Humans
- Lysosomes/metabolism
- Mesoderm/physiology*
- Protein Serine-Threonine Kinases/genetics
- Protein Serine-Threonine Kinases/metabolism
- RNA, Small Interfering/genetics
- RNA, Small Interfering/metabolism
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/metabolism
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Two-Hybrid System Techniques
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rho-Associated Kinases/genetics
- rho-Associated Kinases/metabolism*
- PubMed
- 19509062 Full text @ J. Cell Sci.
Citation
Zhang, Y., Li, X., Qi, J., Wang, J., Liu, X., Zhang, H., Lin, S.C., and Meng, A. (2009) Rock2 controls TGFβ signaling and inhibits mesoderm induction in zebrafish embryos. Journal of Cell Science. 122(Pt 13):2197-2207.
Abstract
The Rho-associated serine/threonine kinases Rock1 and Rock2 play important roles in cell contraction, adhesion, migration, proliferation and apoptosis. Here we report that Rock2 acts as a negative regulator of the TGFbeta signaling pathway. Mechanistically, Rock2 binds to and accelerates the lysosomal degradation of TGFbeta type I receptors internalized from the cell surface in mammalian cells. The inhibitory effect of Rock2 on TGFbeta signaling requires its kinase activity. In zebrafish embryos, injection of rock2a mRNA attenuates the expression of mesodermal markers during late blastulation and blocks the induction of mesoderm by ectopic Nodal signals. By contrast, overexpression of a dominant negative form of zebrafish rock2a, dnrock2a, has an opposite effect on mesoderm induction, suggesting that Rock2 proteins are endogenous inhibitors for mesoderm induction. Thus, our data have unraveled previously unidentified functions of Rock2, in controlling TGFbeta signaling as well as in regulating embryonic patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping