PUBLICATION

Live cell imaging of zebrafish leukocytes

Authors
Hall, C., Flores, M.V., Crosier, K., and Crosier, P.
ID
ZDB-PUB-090424-9
Date
2009
Source
Methods in molecular biology (Clifton, N.J.)   546: 255-271 (Chapter)
Registered Authors
Crosier, Kathy, Crosier, Phil, Flores, Maria, Hall, Chris
Keywords
Zebrafish, Live cell imaging, Neutrophils, Macrophages, Inflammation, Phagocytosis, Tg(lyz:EGFP/DsRED2), Transgenic, pHrodo, Escherichia coli BioParticles
MeSH Terms
  • Animals
  • Cell Compartmentation
  • Disease Models, Animal
  • Genes, Reporter
  • Humans
  • Image Processing, Computer-Assisted/instrumentation
  • Image Processing, Computer-Assisted/methods*
  • Inflammation/immunology
  • Inflammation/metabolism
  • Inflammation/pathology
  • Leukocytes/immunology
  • Leukocytes/metabolism
  • Leukocytes/pathology*
  • Luminescent Proteins
  • Microscopy, Confocal
  • Recombinant Fusion Proteins/biosynthesis
  • Zebrafish/embryology*
  • Zebrafish/immunology*
  • Zebrafish/metabolism
PubMed
19378109 Full text @ Meth. Mol. Biol.
Abstract
Zebrafish are ideally suited for the live imaging of early immune cell compartments. Macrophages that initially appear on the yolk surface prior to the onset of circulation are the first functional immune cells within the embryo, predating the emergence of the first granulocytic cells-the heterophilic neutrophils. Both cell types have been shown in zebrafish to contribute to a robust early innate immune system, capable of clearing systemic infections and participating in wound healing. Early imaging of these cells within zebrafish relied on differential interference contrast (DIC) optics because of their superficial locations in the embryo and the optical transparency of embryonic tissues. Recently, the creation of a number of transgenic reporter lines possessing fluorescently marked myelomonocytic compartments provides the potential to live image these cells during the inflammatory response, in real-time, within a whole animal context. Live imaging during the different stages of inflammation using this expanding library of reporter lines, coupled with the ability to model aspects of human disease in the zebrafish system, have the potential to provide significant insights into inflammation and diseases associated with its dysregulation.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping