PUBLICATION
Effects of Adrenergic Agents on the Expression of Zebrafish (Danio rerio) Vitellogenin Ao1
- Authors
- Yin, N., Jin, X., He, J., and Yin, Z.
- ID
- ZDB-PUB-090422-11
- Date
- 2009
- Source
- Toxicology and applied pharmacology 238(1): 20-26 (Journal)
- Registered Authors
- He, Jiangyan
- Keywords
- Zebrafish, vtgAo1, adrenergic agents, heart, liver
- MeSH Terms
-
- Adrenergic alpha-Agonists/pharmacology*
- Adrenergic alpha-Antagonists/pharmacology
- Adrenergic beta-Agonists/pharmacology*
- Animals
- Enzyme-Linked Immunosorbent Assay
- Gene Expression Regulation/drug effects*
- Isoproterenol/pharmacology
- Liver/metabolism
- Moxisylyte/pharmacology
- Myocardium/metabolism
- Phenylephrine/pharmacology
- RNA, Messenger/drug effects
- RNA, Messenger/metabolism
- Receptors, Estrogen/antagonists & inhibitors
- Tamoxifen/pharmacology
- Vitellogenins/drug effects*
- Vitellogenins/genetics
- Zebrafish
- PubMed
- 19371759 Full text @ Tox. App. Pharmacol.
- CTD
- 19371759
Citation
Yin, N., Jin, X., He, J., and Yin, Z. (2009) Effects of Adrenergic Agents on the Expression of Zebrafish (Danio rerio) Vitellogenin Ao1. Toxicology and applied pharmacology. 238(1):20-26.
Abstract
Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for "reverse triglyceride transportation" similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the beta-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents, but by adrenergic signals as well.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping