PUBLICATION

Determination of mRNA and protein expression patterns in zebrafish

Authors
Christie, E.L., Parslow, A.C., and Heath, J.K.
ID
ZDB-PUB-090106-25
Date
2008
Source
Methods in molecular biology (Clifton, N.J.)   469: 253-272 (Chapter)
Registered Authors
Christie, Elizabeth, Heath, Joan K., Parslow, Adam
Keywords
whole-mount in situ hybridization, riboprobe, whole-mount immunocytochemistry, antibody, vibrating microtome, sections
MeSH Terms
  • Animals
  • Gene Expression*
  • Microinjections/instrumentation
  • Microinjections/methods
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Signal Transduction/physiology
  • Wnt Proteins/antagonists & inhibitors
  • Wnt Proteins/metabolism
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
19109715 Full text @ Meth. Mol. Biol.
Abstract
Optically transparent zebrafish embryos provide an excellent vertebrate model system in which to reveal specific mRNA and protein expression patterns during development. Whole-mount preparations can be used to generate three-dimensional color or fluorescent readouts of the expression pattern of a given gene (or genes), matched with a bright-field image of all the tissues in the developing embryo. Whole-mount mRNA in situhybridization (WISH) has long been the method of choice for revealing gene expression patterns in zebrafish because this method depends only on being able to identify a relatively short region of nucleotide sequence unique for the gene of interest. In contrast, the scarcity of antibodies that are specific to or cross-react with zebrafish proteins has limited the widespread use of immunocyto-chemical applications, though this situation will improve in the future. The elucidation of the specific expression patterns of Wnt pathway genes in zebrafish has made a major contribution to our current understanding of their roles in vertebrate development.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping