PUBLICATION

Meganuclease and transposon mediated transgenesis in medaka

Authors
Grabher, C., and Wittbrodt, J.
ID
ZDB-PUB-071210-11
Date
2007
Source
Genome biology   8(1): S10 (Review)
Registered Authors
Grabher, Clemens, Wittbrodt, Jochen
Keywords
none
MeSH Terms
  • Animals
  • DNA Transposable Elements/genetics*
  • Deoxyribonucleases, Type II Site-Specific/metabolism*
  • Gene Transfer Techniques*
  • Oryzias/genetics*
  • Saccharomyces cerevisiae Proteins
PubMed
18047687 Full text @ Genome Biol.
Abstract
From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping