PUBLICATION
Meganuclease and transposon mediated transgenesis in medaka
- Authors
- Grabher, C., and Wittbrodt, J.
- ID
- ZDB-PUB-071210-11
- Date
- 2007
- Source
- Genome biology 8(1): S10 (Review)
- Registered Authors
- Grabher, Clemens, Wittbrodt, Jochen
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Transposable Elements/genetics*
- Deoxyribonucleases, Type II Site-Specific/metabolism*
- Gene Transfer Techniques*
- Oryzias/genetics*
- Saccharomyces cerevisiae Proteins
- PubMed
- 18047687 Full text @ Genome Biol.
Citation
Grabher, C., and Wittbrodt, J. (2007) Meganuclease and transposon mediated transgenesis in medaka. Genome biology. 8(1):S10.
Abstract
From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping