PUBLICATION

A two-color acid-free cartilage and bone stain for zebrafish larvae

Authors
Walker, M., and Kimmel, C.
ID
ZDB-PUB-070523-23
Date
2007
Source
Biotechnic & histochemistry : official publication of the Biological Stain Commission   82(1): 23-28 (Journal)
Registered Authors
Kimmel, Charles B., Walker, Macie B.
Keywords
alcian blue, alizarin red, PCR genotyping
MeSH Terms
  • Acids
  • Animals
  • Bone and Bones/anatomy & histology*
  • Cartilage/anatomy & histology*
  • Coloring Agents*
  • Histocytochemistry/methods*
  • Larva/anatomy & histology*
  • Magnesium Chloride*
  • Polymerase Chain Reaction
  • Zebrafish
PubMed
17510811 Full text @ Biotech. Histochem.
Abstract
Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping