PUBLICATION

Behavioral phenotyping in zebrafish: comparison of three behavioral quantification methods

Authors
Blaser, R., and Gerlai, R.
ID
ZDB-PUB-061229-28
Date
2006
Source
Behavior research methods   38(3): 456-469 (Journal)
Registered Authors
Gerlai, Robert T.
Keywords
none
MeSH Terms
  • Aggression/physiology*
  • Analysis of Variance
  • Animals
  • Behavior, Animal/physiology*
  • Behavioral Sciences/methods
  • Escape Reaction/physiology
  • Exploratory Behavior/physiology*
  • Female
  • Male
  • Motor Activity/physiology
  • Phenotype*
  • Reference Values
  • Social Behavior*
  • Statistics, Nonparametric
  • Zebrafish
PubMed
17186756 Full text @ Behav. Res. Methods
Abstract
The zebrafish has been popular in developmental biology and genetics, but its brain function has rarely been studied. High-throughput screening of mutation or drug-induced changes in brain function requires simple and automatable behavioral tests. This article compares three behavioral quantification methods in four simple behavioral paradigms that test a range of characteristics of adult zebrafish, including novelty-induced responses, social behavior, aggression, and predator-model-induced responses. Two quantification methods, manual recording and computerized videotracking of location and activity, yielded very similar results, suggesting that automated videotracking reliably measures activity parameters and will allow high-throughput screening. However, observation-based event recording of posture patterns was found generally not to correlate with videotracking measures, suggesting that further refinement of automated behavior quantification may be considered.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping