PUBLICATION

Diversity of zebrafish peripheral oscillators revealed by luciferase reporting

Authors
Kaneko, M., Hernandez-Borsetti, N., and Cahill, G.M.
ID
ZDB-PUB-060921-18
Date
2006
Source
Proceedings of the National Academy of Sciences of the United States of America   103(39): 14614-14619 (Journal)
Registered Authors
Cahill, Greg, Kaneko, Maki
Keywords
circadian, period3, bioluminescence, temperature compensation
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Biological Clocks/physiology*
  • Biological Clocks/radiation effects
  • Circadian Rhythm/physiology
  • Circadian Rhythm/radiation effects
  • Darkness
  • Genes, Reporter/genetics*
  • Heart/physiology
  • Light
  • Luciferases/genetics
  • Luciferases/metabolism*
  • Luminescent Proteins/metabolism
  • Nuclear Proteins/metabolism
  • Organ Specificity
  • Period Circadian Proteins
  • Spleen/physiology
  • Temperature
  • Transcription Factors/metabolism
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins
PubMed
16973754 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
In various multicellular organisms, circadian clocks are present not only in the central nervous system, but also in peripheral organs and tissues. In mammals peripheral oscillators are not directly responsive to light, but are entrained by the central oscillator in the suprachiasmatic nucleus. These individual oscillators are diverse in their free-running periods and phases. In contrast, cultured peripheral tissues and cell lines from zebrafish are not only rhythmic, but can also be directly entrained by light. Because of the convenience of studying rhythms in cultured cells, however, little has been known about properties of individual oscillators in intact zebrafish. Here, we show the remarkable diversity and consistency of oscillator properties in various peripheral organs and tissues from the period3-luciferase (per3-luc) transgenic zebrafish. Tissue-dependent differences were found in free-running period, phase, response to light, and temperature compensation. Furthermore, cycling amplitudes were reduced at lower temperatures in some, but not all, of the organs tested. Finally, we found that per3-luc rhythms can free run in both constant dark and constant light with remarkably similar amplitudes, phases, and periods, despite the fact that the mRNA of per2 and per1 has been shown not to oscillate in constant light.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping