PUBLICATION
Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development
- Authors
- Liebling, M., Forouhar, A.S., Wolleschensky, R., Zimmermann, B., Ankerhold, R., Fraser, S.E., Gharib, M., and Dickinson, M.E.
- ID
- ZDB-PUB-060825-16
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(11): 2940-2948 (Journal)
- Registered Authors
- Forouhar, Arian S., Fraser, Scott E., Liebling, Michael
- Keywords
- Zebrafish, heart development, hemodynamics, confocal microscopy, image reconstruction, 4D imaging
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/chemistry
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology
- Green Fluorescent Proteins/analysis
- Green Fluorescent Proteins/genetics
- Heart/embryology*
- Heart/physiology
- Imaging, Three-Dimensional/methods*
- Microscopy, Confocal/methods*
- Myocardial Contraction/physiology*
- Myocardium/chemistry
- Myocardium/cytology
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 16921497 Full text @ Dev. Dyn.
Citation
Liebling, M., Forouhar, A.S., Wolleschensky, R., Zimmermann, B., Ankerhold, R., Fraser, S.E., Gharib, M., and Dickinson, M.E. (2006) Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(11):2940-2948.
Abstract
We report an accurate method for studying the functional dynamics of the beating embryonic zebrafish heart. The fast cardiac contraction rate and the high velocity of blood cells have made it difficult to study cellular and subcellular events relating to heart function in vivo. We have devised a dynamic three-dimensional acquisition, reconstruction, and analysis procedure by combining (1) a newly developed confocal slit-scanning microscope, (2) novel strategies for collecting and synchronizing cyclic image sequences to build volumes with high temporal and spatial resolution over the entire depth of the beating heart, and (3) data analysis and reduction protocols for the systematic extraction of quantitative information to describe phenotype and function. We have used this approach to characterize blood flow and heart efficiency by imaging fluorescent protein-expressing blood and endocardial cells as the heart develops from a tube to a multichambered organ. The methods are sufficiently robust to image tissues within the heart at cellular resolution over a wide range of ages, even when motion patterns are only quasiperiodic. These tools are generalizable to imaging and analyzing other cyclically moving structures at microscopic scales.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping