PUBLICATION
Zebrafish assays for drug toxicity screening
- Authors
- Rubinstein, A.L.
- ID
- ZDB-PUB-060731-11
- Date
- 2006
- Source
- Expert opinion on drug metabolism & toxicology 2(2): 231-40 (Review)
- Registered Authors
- Rubinstein, Amy
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Drug Evaluation, Preclinical/methods*
- Drug Evaluation, Preclinical/trends
- Forecasting
- Humans
- Models, Animal*
- Toxicity Tests/methods*
- Toxicity Tests/trends
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 16866609 Full text @ Expert. Opin. Drug Metab. Toxicol.
Citation
Rubinstein, A.L. (2006) Zebrafish assays for drug toxicity screening. Expert opinion on drug metabolism & toxicology. 2(2):231-40.
Abstract
Zebrafish are vertebrate organisms that are of growing interest for preclinical drug discovery applications. Zebrafish embryos develop most of the major organ systems present in mammals, including the cardiovascular, nervous and digestive systems, in < 1 week. Additional characteristics that make them advantageous for compound screening are their small size, transparency and ability to absorb compounds through the water. Furthermore, gene function analysis with antisense technology is now routine procedure. Thus, it is relatively simple to assess whether compounds or gene knockdowns cause toxic effects in zebrafish. Assays are being developed to exploit the unique characteristics of zebrafish for pharmacological toxicology. This review discusses assays that may be used to assess in vivo toxicity and provides examples of compounds known to be toxic to humans that have been demonstrated to function similarly in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping