PUBLICATION

Young thrombocytes initiate the formation of arterial thrombi in zebrafish

Authors
Thattliyath, B., Cykowski, M., and Jagadeeswaran, P.
ID
ZDB-PUB-050318-18
Date
2005
Source
Blood   106(1): 118-124 (Journal)
Registered Authors
Jagadeeswaran, Pudur
Keywords
none
MeSH Terms
  • Animals
  • Arteries
  • Blood Platelets/cytology*
  • Blood Platelets/physiology*
  • Carbocyanines
  • Cellular Senescence
  • Fluorescent Dyes
  • Hemostasis/physiology*
  • Humans
  • Thrombosis/physiopathology*
  • Zebrafish
PubMed
15769888 Full text @ Blood
Abstract
The zebrafish system is an excellent vertebrate genetic model to study hemostasis and thrombosis because saturation mutagenesis screens can identify novel genes that play a role in this vital physiological pathway. To study hemostatic mutations, it is important to understand the physiology of zebrafish hemostasis and thrombosis. Previously, we identified zebrafish thrombocytes and have shown that they participate in arterial thrombus formation. Here, we recognized two populations of thrombocytes distinguishable by DiI-C18 (DiI) staining. DiI+ thrombocytes have a high density of adhesive receptors and are functionally more active than DiI- thrombocytes. We classified DiI+ thrombocytes as young and DiI- thrombocytes as mature thrombocytes. We found young thrombocytes and mature thrombocytes each formed independent clusters and that young thrombocytes clustered first. We have also shown that young thrombocytes initiate arterial thrombus formation. We propose that due to the increased adhesive receptor density on young thrombocytes, they adhere first to the sub-endothelial matrix, get activated rapidly, release agonists and recruit more young thrombocytes which further release more agonists. This increase in agonists activates the less active mature thrombocytes, drawing them to the growing thrombus. Since arterial thrombus formation is a fundamental hemostatic event, the above mechanism may be conserved in mammals and may open new avenues for prevention of arterial thrombosis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping