PUBLICATION

Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7) from zebrafish (Danio rerio)

Authors
Liu, R.Z., Denovan-Wright, E.M., and Wright, J.M.
ID
ZDB-PUB-030224-2
Date
2003
Source
European journal of biochemistry   270(4): 715-725 (Journal)
Registered Authors
Wright, Jonathan M.
Keywords
none
MeSH Terms
  • 5' Untranslated Regions/genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/metabolism
  • Carrier Proteins/genetics*
  • Carrier Proteins/metabolism
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA Primers/chemistry
  • Fatty Acid-Binding Proteins
  • Fatty Acids/genetics
  • Fatty Acids/metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Linkage/genetics
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Nerve Tissue Proteins*
  • Promoter Regions, Genetic/genetics
  • RNA, Messenger/biosynthesis*
  • Radiation Hybrid Mapping
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Tumor Suppressor Proteins*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins*
PubMed
12581211 Full text @ Eur. J. Biochem.
Abstract
The brain fatty acid-binding protein (B-FABP) is involved in brain development and adult neurogenesis. We have determined the sequence of the gene encoding the B-FABP in zebrafish. The zebrafish B-FABP gene spans 2370 bp and contains four exons interrupted by three introns. The coding sequence of zebrafish B-FABP gene is identical to its cDNA sequence and the coding capacity of each exon is the same as that for the human and mouse B-FABP genes. A 1249 bp sequence 5' upstream of exon 1 of the zebrafish B-FABP gene was cloned and sequenced. Several brain development/growth-associated transcription factor binding elements, including POU-domain binding elements and the proposed lipogenic- associated transcription factor NF-Y elements, were found within the 5' region of the B-FABP gene. RT-PCR analysis using mRNA extracted from different tissues of adult zebrafish demonstrated that the zebrafish B- FABP mRNA was predominant in brain with lower levels in liver, testis and intestine, but not in ovary, skin, heart, kidney and muscle. Quantitative RT-PCR revealed a similar tissue-specific distribution for zebrafish B-FABP mRNA except that very low levels of B-FABP mRNA, normalized to beta-actin mRNA, were detected in the heart and muscle RNA , but not in liver RNA. Zebrafish B-FABP mRNA was detected by RT-PCR in embryos beyond 12 h postfertilization, suggesting a correlation of zebrafish B-FABP mRNA expression with early brain development. Radiation hybrid mapping assigned the zebrafish B-FABP gene to linkage group 17. Conserved syntenies of the zebrafish B-FABP gene and the human and mouse orthologous B-FABP genes were observed by comparative genomic analysis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping