PUBLICATION

Mammalian germ-line transgenesis by transposition

Authors
Dupuy, A.J., Clark, K., Carlson, C.M., Fritz, S., Davidson, A.E., Markley, K.M., Finley, K., Fletcher, C.F., Ekker, S.C., Hackett, P.B., Horn, S., and Largaespada, D.A.
ID
ZDB-PUB-020805-4
Date
2002
Source
Proceedings of the National Academy of Sciences of the United States of America   99(7): 4495-4499 (Journal)
Registered Authors
Clark, Karl, Ekker, Stephen C., Hackett, Perry B.
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Transposable Elements*
  • Molecular Sequence Data
  • Mutagenesis, Insertional*
  • Transposases/genetics
  • Transposases/physiology
PubMed
11904379 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping