PUBLICATION

Some distinctive features of zebrafish myogenesis based on unexpected distributions of the muscle cytoskeletal proteins actin, myosin, desmin, alpha-actinin, troponin and titin

Authors
Costa, M.L., Escaleira, R.C., Rodrigues, V.B., Manasfi, M., and Mermelstein, C.S.
ID
ZDB-PUB-020730-10
Date
2002
Source
Mechanisms of Development   116(1-2): 95-104 (Journal)
Registered Authors
Costa, Manoel Luis, Escaleira, Roberta, Mermelstein, Claudia
Keywords
myogenesis; myofibrillogenesis; actin; myosin; desmin; a-actinin; titin; troponin; zebrafish
MeSH Terms
  • Actinin/metabolism
  • Actins/metabolism
  • Animals
  • Connectin
  • Cytoskeletal Proteins/metabolism*
  • Desmin/metabolism
  • Microscopy, Fluorescence
  • Muscle Development/physiology*
  • Muscle Proteins/metabolism*
  • Myosins/metabolism
  • Protein Kinases/metabolism
  • Troponin/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
PubMed
12128209 Full text @ Mech. Dev.
Abstract
The current myofibrillogenesis model is based mostly on in vitro cell cultures and on avian and mammalian embryos in situ. We followed the expression of actin, myosin, desmin, alpha-actinin, titin, and troponin using immunofluorescence microscopy of zebrafish (Danio rerio) embryos. We could see young mononucleated myoblasts with sharp striations. The striations were positive for all the sarcomeric proteins. Desmin distribution during muscle maturation changes from dispersed aggregates to a perinuclear concentration to striated afterwards. We could not observe desmin-positive, myofibrillar-proteins-negative cells, and we could not find any non-striated distribution of sarcomeric proteins, such as stress fiber-like structures. Some steps, like fusion before striation, seem to be different in the zebrafish when compared with the previously described myogenesis sequences.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping