PUBLICATION

Structure and macromolecular composition of the zebrafish egg chorion

Authors
Bonsignorio, D., Perego, L., Del Giacco, L., and Cotelli, F.
ID
ZDB-PUB-020124-1
Date
1996
Source
Zygote (Cambridge, England)   4(2): 101-108 (Journal)
Registered Authors
Bonsignorio, Daniele, Cotelli, Franco, Del Giacco, Luca
Keywords
none
MeSH Terms
  • Amino Acids/analysis
  • Amino Acids/chemistry
  • Animals
  • Chorion/chemistry*
  • Chorion/ultrastructure*
  • Chromatography, High Pressure Liquid
  • Concanavalin A/chemistry
  • Concanavalin A/metabolism
  • Egg Proteins/chemistry*
  • Egg Proteins/metabolism
  • Electrophoresis, Polyacrylamide Gel/methods
  • Female
  • Galanthus
  • Glycoproteins/chemistry
  • Glycoproteins/metabolism
  • Glycosylation
  • Microscopy, Electron
  • Molecular Sequence Data
  • Oocytes/ultrastructure
  • Ovum/ultrastructure
  • Plant Lectins
  • Zebrafish*
PubMed
8913023 Full text @ Zygote
Abstract
The chorion is the acellular envelope surrounding mature eggs of teleostean fish. The macromolecular composition of the zebrafish (Danio rerio) egg chorion, organised as a three-layered structure, has been analysed. SDS-PAGE analysis, under reducing conditions, of isolated and purified chorions revealed a reproducible pattern of four major polypeptides (116, 97, 50 and 43 kDa) and several minor bands. Lectin binding assays showed that both the 116 kDa and 50 kDa proteins were recognised by concanavalin agglutinin (Con A), Galanthus nivalis agglutinin (GNA), Sambucus nigra bark agglutinin (SNA) and Ricinus communis agglutinin (RCA 120), suggesting that these polypeptides are N-linked glycoproteins. By contrast, neither the 97 kDa nor the 43 kDa polypeptides were stained by these lectins, indicating that these polypeptides are not glycosylated. Amino acid analysis also showed significant differences in the average content of some amino acids, for example serine and prol! ine, when compared with previous reports.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping