PUBLICATION

A neuropeptide Y receptor Y1-subfamily gene from an agnathan, the European river lamprey

Authors
Salaneck, E., Fredriksson, R., Larson, E.T., Conlon, J.M., and Larhammar, D.
ID
ZDB-PUB-020102-7
Date
2001
Source
European journal of biochemistry   268(23): 6146-6154 (Journal)
Registered Authors
Fredriksson, Robert, Larhammar, Dan, Larson, Earl T., Salaneck, Erik
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers/genetics
  • Evolution, Molecular
  • Gene Duplication
  • Kinetics
  • Lampreys/genetics*
  • Lampreys/metabolism
  • Ligands
  • Models, Genetic
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, Neuropeptide Y/genetics*
  • Receptors, Neuropeptide Y/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
PubMed
11733009 Full text @ Eur. J. Biochem.
Abstract
We report here the isolation and functional expression of a neuropeptide Y (NPY) receptor from the river lamprey, Lampetra fluviatilis. The receptor displays approximately 50% amino-acid sequence identity to all previously cloned Y1-subfamily receptors including Y1, Y4, and y6 and the teleost subtypes Ya, Yb and Yc. Phylogenetic analyses point to a closer relationship with Y4 and Ya/b/c suggesting that the lamprey receptor could possibly represent a pro-orthologue of some or all of those gnathostome receptors. Our results support the notion that the Y1 subfamily increased in number by genome or large-scale chromosome duplications, one of which may have taken place prior to the divergence of lampreys and gnathostomes whereas the second duplication probably occurred in the gnathostome lineage after this split. Functional expression of the lamprey receptor in a cell line facilitated specific binding of the three endogenous lamprey peptides NPY, peptide YY and peptide MY with picomolar affinities. Binding studies with a large panel of NPY analogues revealed indiscriminate binding properties similar to those of another nonselective Y1-subfamily receptor, zebrafish Ya. RT-PCR detected receptor mRNA in the central nervous system as well as in several peripheral organs suggesting diverse functions. This lamprey receptor is evolutionarily the most distant NPY receptor that clearly belongs to the Y1 subfamily as defined in mammals, which shows that subtypes Y2 and Y5 arose even earlier in evolution.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping