PUBLICATION

Target selection for Danio rerio functional genomics

Authors
Klee, E.W., Ekker, S.C., and Ellis, L.B.M.
ID
ZDB-PUB-010917-3
Date
2001
Source
Genesis (New York, N.Y. : 2000)   30(3): 123-125 (Journal)
Registered Authors
Ekker, Stephen C., Klee, Eric W.
Keywords
functional genomics; secreted proteins; zebrafish
MeSH Terms
  • Animals
  • Databases as Topic
  • Expressed Sequence Tags
  • Genomics*
  • Protein Sorting Signals/genetics
  • Protein Sorting Signals/physiology*
  • Proteome*
  • Sequence Alignment
  • Sequence Homology
  • Software
  • Zebrafish/genetics*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
11477688 Full text @ Genesis
Abstract
Secreted proteins are of interest as targets for morpholino technology since they frequently mediate cell-cell interactions. Most are identified through the presence of an N-terminal signal sequence. Proteins from the zebrafish Danio rerio containing such signal sequences will be easy to detect with the publication of the complete genome, expected by the end of 2003 (Sanger Centre, 2000). Until then, most zebrafish proteins are only available as translated Expressed Sequence Tags (ESTs) that often lack N-terminal residues. Interim innovative techniques for the identification of EST-derived proteins predicted to contain signal sequences are needed. Here we describe the use of genetic homology among eukaryotes as a basis for selecting a subset of zebrafish sequences with high probability of encoding secreted proteins. We estimate there are 1,000-2,000 gene families encoding these proteins in the zebrafish genome.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping