PUBLICATION

Double in situ hybridization techniques in zebrafish

Authors
Jowett, T.
ID
ZDB-PUB-010424-1
Date
2001
Source
Methods (San Diego, Calif.)   23(4): 345-358 (Review)
Registered Authors
Jowett, Trevor
Keywords
none
MeSH Terms
  • Alkaline Phosphatase/metabolism
  • Animals
  • Fluorescent Antibody Technique, Indirect
  • Fluorescent Dyes/metabolism
  • Gene Expression
  • Horseradish Peroxidase/metabolism
  • In Situ Hybridization/methods*
  • Nucleic Acid Hybridization
  • RNA, Antisense/metabolism
  • RNA, Messenger/metabolism
  • Zebrafish
PubMed
11316436 Full text @ Methods
Abstract
Over recent years many genes expressed in zebrafish embryos have been cloned and a first step in their analysis is the determination of the spatial and temporal expression patterns of their transcripts by in situ hybridization. It is often necessary to relate the expression pattern to that of other genes expressed at the same period of development. Transcripts from different genes may be expressed in complementary or overlapping domains. The pattern of expression of different genes can be related by performing in situ hybridization on consecutive sections of tissue but it is also possible to perform hybridizations with two probes and to visualize the signals separately in the same tissue. Occasionally it is also possible to combine in situ hybridization with immunocytochemistry to localize tissue-specific antigens. Methods developed for performing these types of analyses in zebrafish are described in this article.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping