Imaging of Zebrafish prechordal plate using TipTracker with the LavisionBiotech TriM Scope II.

(A) Schematic representation of the shield stage of a six hpf gsc::mEGFP Zebrafish embryo. Membrane bound EGFP is expressed in the prechordal plate (ppl) cells that form the shield (green). (B) Maximum intensity projections of three time points out of 78 show the gsc::mEGFP expression over time. (C) A transversal section (3.40 μm width maximum intensity projection) shows ppl cells ingression and subsequent migration. See also Video 8. (D) Schematic representation of gsc::mEGFP expressing cells ingression and migration between shield stage and 90% epiboly stage (9 h post fertilization). A stack of 50 images (x/y/z: 1024 × 1024 × 50 pixels, voxelsize: 0.342 × 0.342 × 3.0 μm³) was captured every 2 min 15 s for a period of 2 h 50 min using the Zeiss Plan-Apochromat 20x/1.0 air objective lens. Time 0 corresponds to six hpf. Scale bars: (B, C) 50 μm.