PUBLICATION
Derivation and characterization of a zebrafish liver cell line
- Authors
- Ghosh, C., Zhou, Y.L., and Collodi, P.
- ID
- ZDB-PUB-961101-36
- Date
- 1994
- Source
- Cell biology and toxicology 10: 167-176 (Journal)
- Registered Authors
- Collodi, Paul, Ghosh, Chandramallika
- Keywords
- none
- MeSH Terms
-
- Albumins/biosynthesis
- Alkaline Phosphatase/biosynthesis
- Animals
- Aspartate Aminotransferases/biosynthesis
- Cell Division
- Cell Line*
- Culture Media
- Glucose-6-Phosphatase/biosynthesis
- Karyotyping
- Liver/cytology*
- Liver/metabolism
- Zebrafish*
- PubMed
- 7994634 Full text @ Cell Biol. Toxicol.
Citation
Ghosh, C., Zhou, Y.L., and Collodi, P. (1994) Derivation and characterization of a zebrafish liver cell line. Cell biology and toxicology. 10:167-176.
Abstract
ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number = 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping