PUBLICATION
Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping: A pipeline for whole-brain structural and activity analyses
- Authors
- Jin, D.S., Neelakantan, U., Lacadie, C.M., Chen, T., Rooney, B., Liu, Y., Wu, W., Wang, Z., Papademetris, X., Hoffman, E.J.
- ID
- ZDB-PUB-231029-68
- Date
- 2023
- Source
- STAR protocols 4: 102647102647 (Journal)
- Registered Authors
- Chen, Tianying, Hoffman, Ellen
- Keywords
- Computer Sciences, Genetics, Microscopy, Model Organisms, Neuroscience
- MeSH Terms
-
- Animals
- Brain*/diagnostic imaging
- Brain Mapping
- Humans
- Larva
- Phenotype
- Zebrafish*
- PubMed
- 37897734 Full text @ STAR Protoc
Citation
Jin, D.S., Neelakantan, U., Lacadie, C.M., Chen, T., Rooney, B., Liu, Y., Wu, W., Wang, Z., Papademetris, X., Hoffman, E.J. (2023) Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping: A pipeline for whole-brain structural and activity analyses. STAR protocols. 4:102647102647.
Abstract
Here, we present Brain Registration and Evaluation for Zebrafish (BREEZE)-mapping, a user-friendly pipeline for the registration and analysis of whole-brain images in larval zebrafish. We describe steps for pre-processing, registration, quantification, and visualization of whole-brain phenotypes in zebrafish mutants of genes associated with neurodevelopmental and neuropsychiatric disorders. By utilizing BioImage Suite Web, an open-source software package originally developed for processing human brain imaging data, we provide a highly accessible whole-brain mapping protocol developed for users with general computational proficiency. For complete details on the use and execution of this protocol, please refer to Weinschutz Mendes et al. (2023).1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping