PUBLICATION
Caspase-3-Triggered Intracellular Gadolinium Nanoparticle Formation for T1-Weighted Magnetic Resonance Imaging of Apoptosis In Vivo
- Authors
- Xu, H.D., Cheng, X., Sun, X., Chen, P., Zhan, W., Liu, X., Wang, X., Hu, B., Liang, G.
- ID
- ZDB-PUB-230627-38
- Date
- 2023
- Source
- Nano Letters 23(13): 6178-6183 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- Apoptosis, CBT-Cys click reaction, Caspase-3, Contrast agent, T1-weighted MRI
- MeSH Terms
-
- Animals
- Apoptosis
- Caspase 3
- Contrast Media
- Gadolinium*
- Magnetic Resonance Imaging/methods
- Nanoparticles*
- Zebrafish
- PubMed
- 37363812 Full text @ Nano Lett.
Citation
Xu, H.D., Cheng, X., Sun, X., Chen, P., Zhan, W., Liu, X., Wang, X., Hu, B., Liang, G. (2023) Caspase-3-Triggered Intracellular Gadolinium Nanoparticle Formation for T1-Weighted Magnetic Resonance Imaging of Apoptosis In Vivo. Nano Letters. 23(13):6178-6183.
Abstract
Apoptosis, with a hallmark of upregulated protease Caspase-3, has been frequently imaged with various probes to reveal the therapeutic efficiencies of different drugs. However, activatable molecular probes with programmable self-assembling behaviors that enable enhanced T1-weighted magnetic resonance imaging (MRI) of apoptosis remain scarce. Herein, taking advantage of a CBT-Cys click reaction, we rationally designed a Caspase-3-activatable self-assembling probe Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys(DOTA(Gd))-CBT (DEVDCS-Gd-CBT) for apoptosis imaging in vivo. After Caspase-3 cleavage in apoptotic cells, DEVDCS-Gd-CBT underwent CBT-Cys click reaction to form a cyclic dimer, which self-assembled into Gd nanoparticles. With this probe, enhanced T1-weighted MR images of apoptosis were achieved at low magnetic fields in vitro, in cis-dichlorodiamineplatinum-induced apoptotic cells and in tail-amputation-simulated apoptotic zebrafish. We anticipate that the smart probe DEVDCS-Gd-CBT could be applied for T1-weighted MRI of apoptosis-related diseases in the clinic in the future.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping