PUBLICATION
Lack of Paxillin phosphorylation promotes single-cell migration in vivo
- Authors
- Xue, Q., Varady, S.R.S., Waddell, T.Q.A., Roman, M.R., Carrington, J., Roh-Johnson, M.
- ID
- ZDB-PUB-230202-18
- Date
- 2023
- Source
- The Journal of cell biology 222(3): (Journal)
- Registered Authors
- Roh-Johnson, Minna, Varady, Sophia, Waddell, Trinity, Xue, Qian
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Movement*/physiology
- Focal Adhesion Protein-Tyrosine Kinases/metabolism
- Focal Adhesions*/metabolism
- Mice
- Paxillin*/genetics
- Paxillin*/metabolism
- Phosphorylation
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 36723624 Full text @ J. Cell Biol.
Citation
Xue, Q., Varady, S.R.S., Waddell, T.Q.A., Roman, M.R., Carrington, J., Roh-Johnson, M. (2023) Lack of Paxillin phosphorylation promotes single-cell migration in vivo. The Journal of cell biology. 222(3):.
Abstract
Focal adhesions are structures that physically link the cell to the extracellular matrix for cell migration. Although cell culture studies have provided a wealth of information regarding focal adhesion biology, it is critical to understand how focal adhesions are dynamically regulated in their native environment. We developed a zebrafish system to visualize focal adhesion structures during single-cell migration in vivo. We find that a key site of phosphoregulation (Y118) on Paxillin exhibits reduced phosphorylation in migrating cells in vivo compared to in vitro. Furthermore, expression of a non-phosphorylatable version of Y118-Paxillin increases focal adhesion disassembly and promotes cell migration in vivo, despite inhibiting cell migration in vitro. Using a mouse model, we further find that the upstream kinase, focal adhesion kinase, is downregulated in cells in vivo, and cells expressing non-phosphorylatable Y118-Paxillin exhibit increased activation of the CRKII-DOCK180/RacGEF pathway. Our findings provide significant new insight into the intrinsic regulation of focal adhesions in cells migrating in their native environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping