PUBLICATION
KHz-rate volumetric voltage imaging of the whole Zebrafish heart
- Authors
- Sacconi, L., Silvestri, L., Rodríguez, E.C., Armstrong, G.A.B., Pavone, F.S., Shrier, A., Bub, G.
- ID
- ZDB-PUB-221126-1
- Date
- 2022
- Source
- Biophysical reports 2: 100046100046 (Journal)
- Registered Authors
- Armstrong, Gary A.B.
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 36425080 Full text @ Biophys Rep (N Y)
Citation
Sacconi, L., Silvestri, L., Rodríguez, E.C., Armstrong, G.A.B., Pavone, F.S., Shrier, A., Bub, G. (2022) KHz-rate volumetric voltage imaging of the whole Zebrafish heart. Biophysical reports. 2:100046100046.
Abstract
Fast volumetric imaging is essential for understanding the function of excitable tissues such as those found in the brain and heart. Measuring cardiac voltage transients in tissue volumes is challenging, especially at the high spatial and temporal resolutions needed to give insight to cardiac function. We introduce a new imaging modality based on simultaneous illumination of multiple planes in the tissue and parallel detection with multiple cameras, avoiding compromises inherent in any scanning approach. The system enables imaging of voltage transients in situ, allowing us, for the first time to our knowledge, to map voltage activity in the whole heart volume at KHz rates. The high spatiotemporal resolution of our method enabled the observation of novel dynamics of electrical propagation through the zebrafish atrioventricular canal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping