PUBLICATION
Oxytetracycline induces anxiety-like behavior in adult zebrafish
- Authors
- Gusso, D., Altenhofen, S., Fritsch, P.M., Rübensam, G., Bonan, C.D.
- ID
- ZDB-PUB-210609-15
- Date
- 2021
- Source
- Toxicology and applied pharmacology 426: 115616 (Journal)
- Registered Authors
- Bonan, Carla Denise
- Keywords
- Anxiety, Behavior, Cortisol, Oxytetracycline, Zebrafish
- MeSH Terms
-
- Animals
- Anti-Anxiety Agents/therapeutic use
- Anti-Bacterial Agents/adverse effects*
- Anxiety/chemically induced*
- Anxiety/drug therapy
- Aquaculture
- Behavior, Animal/drug effects*
- Clonazepam/therapeutic use
- Female
- GABA Modulators/therapeutic use
- Hydrocortisone/metabolism
- Male
- Oxytetracycline/adverse effects*
- Zebrafish*
- PubMed
- 34102243 Full text @ Tox. App. Pharmacol.
Citation
Gusso, D., Altenhofen, S., Fritsch, P.M., Rübensam, G., Bonan, C.D. (2021) Oxytetracycline induces anxiety-like behavior in adult zebrafish. Toxicology and applied pharmacology. 426:115616.
Abstract
Oxytetracycline (OTC) is one of the broad-spectrum antibiotics widely used for the treatment of fish-farm infection. Considering that behavior is directly related to reproduction, individual fitness, and survival, it is important to evaluate the impact of antibiotics on the behavioral repertoire in fish. Zebrafish (Danio rerio) presents a well-described behavioral repertoire to reliably demonstrate complex responses to chemical compound exposure. This work aims to identify the role of OTC in comprehensive behavioral parameters and whole-body cortisol levels in adult zebrafish. Here we report that OTC exposure (10, 20, and 100 mg/L) induces an anxiogenic-like phenotype in the novel tank test. OTC exposure also changes the behavior of social interaction with a shoal of unknown zebrafish - characterized as a stimulus group. Zebrafish exposed to OTC (10 mg/L) remains a longer period in the stimulus zone when compared to the control group. Clonazepam (0.006 mg/L) was able to reverse anxiogenic-like behavior and the changes in social behavior induced by OTC. We also demonstrated that cortisol levels were significantly decreased after exposure to OTC (10, 20, and 100 mg/L), which were not reversed by clonazepam. These findings highlight the growing utility of zebrafish as a model to understand the impact of antibiotics on behavior and their underlying mechanisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping