PUBLICATION
The FOXK1-CCDC43 Axis Promotes the Invasion and Metastasis of Colorectal Cancer Cells
- Authors
- Wang, J., Liu, G., Liu, M., Xiang, L., Xiao, Y., Zhu, H., Wu, X., Peng, Y., Zhang, W., Jiang, P., Li, A., Nan, Q., Chen, Y., Chen, C., Cheng, T., Liu, S., Wang, J.
- ID
- ZDB-PUB-181219-15
- Date
- 2018
- Source
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 51: 2547-2563 (Journal)
- Registered Authors
- Peng, Ying
- Keywords
- CCDC43, Colorectal cancer, Epithelial-mesenchymal transition, FOXK1, Invasion, Metastasis
- MeSH Terms
-
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Colorectal Neoplasms/diagnosis
- Colorectal Neoplasms/genetics*
- Colorectal Neoplasms/metabolism
- Colorectal Neoplasms/pathology
- Epithelial-Mesenchymal Transition
- Forkhead Transcription Factors/analysis
- Forkhead Transcription Factors/genetics*
- Forkhead Transcription Factors/metabolism
- Gene Expression Regulation, Neoplastic*
- Humans
- Neoplasm Invasiveness/diagnosis
- Neoplasm Invasiveness/genetics*
- Neoplasm Invasiveness/pathology
- Neoplasm Proteins/analysis
- Neoplasm Proteins/genetics*
- Neoplasm Proteins/metabolism
- Prognosis
- Up-Regulation
- PubMed
- 30562730 Full text @ Cell Physiol. Biochem.
Citation
Wang, J., Liu, G., Liu, M., Xiang, L., Xiao, Y., Zhu, H., Wu, X., Peng, Y., Zhang, W., Jiang, P., Li, A., Nan, Q., Chen, Y., Chen, C., Cheng, T., Liu, S., Wang, J. (2018) The FOXK1-CCDC43 Axis Promotes the Invasion and Metastasis of Colorectal Cancer Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 51:2547-2563.
Abstract
Background/aims The CCDC43 gene is conserved in human, rhesus monkey, mouse and zebrafish. Bioinformatics studies have demonstrated the abnormal expression of CCDC43 gene in colorectal cancer (CRC). However, the role and molecular mechanism of CCDC43 in CRC remain unknown.
Methods The functional role of CCDC43 and FOXK1 in epithelial-mesenchymal transition (EMT) was determined using immunohistochemistry, flow cytometry, western blot, EdU incorporation, luciferase, chromatin Immunoprecipitation (ChIP) and cell invasion assays.
Results The CCDC43 gene was overexpressed in human CRC. High expression of CCDC43 protein was associated with tumor progression and poor prognosis in patients with CRC. Moreover, the induction of EMT by CCDC43 occurred through TGF-β signaling. Furthermore, a positive correlation between the expression patterns of CCDC43 and FOXK1 was observed in CRC cells. Promoter assays demonstrated that FOXK1 directly bound and activated the human CCDC43 gene promoter. In addition, CCDC43 was necessary for FOXK1- mediated EMT and metastasis in vitro and vivo. Taken together, this work identified that CCDC43 promoted EMT and was a direct transcriptional target of FOXK1 in CRC cells.
Conclusion FOXK1-CCDC43 axis might be helpful to develop the drugs for the treatment of CRC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping