PUBLICATION
Lipid Bilayer-Coated Mesoporous Silica Nanoparticles Carrying Bovine Hemoglobin towards an Erythrocyte Mimic
- Authors
- Tu, J., Bussmann, J., Du, G., Gao, Y., Bouwstra, J.A., Kros, A.
- ID
- ZDB-PUB-180324-3
- Date
- 2018
- Source
- International journal of pharmaceutics 543(1-2): 169-178 (Journal)
- Registered Authors
- Bussmann, Jeroen
- Keywords
- Erythrocyte mimic, Hemoglobin, Lipid bilayer, Mesoporous silica nanoparticles, Zebrafish embryos
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/metabolism
- Blood Circulation
- Cattle
- Drug Liberation
- Embryo, Nonmammalian/metabolism
- Erythrocytes
- Hemoglobins/administration & dosage*
- Hemoglobins/chemistry
- Lipid Bilayers/administration & dosage*
- Lipid Bilayers/chemistry
- Nanoparticles/administration & dosage*
- Nanoparticles/chemistry
- Porosity
- Silicon Dioxide/administration & dosage*
- Silicon Dioxide/chemistry
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 29567198 Full text @ Int J Pharm
Citation
Tu, J., Bussmann, J., Du, G., Gao, Y., Bouwstra, J.A., Kros, A. (2018) Lipid Bilayer-Coated Mesoporous Silica Nanoparticles Carrying Bovine Hemoglobin towards an Erythrocyte Mimic. International journal of pharmaceutics. 543(1-2):169-178.
Abstract
Hemoglobin (Hb)-loaded mesoporous silica nanoparticles (MSNs) coated with a lipid bilayer (LB-MSNs) were investigated as an erythrocyte mimic. MSNs with a large average pore size (10 nm) act as a rigid core and provide a protective environment for Hb encapsulated inside the pores. The colloidal stability of Hb-loaded MSNs was enhanced upon the application of a lipid bilayer, through fusion of PEGylated liposomes onto the exterior surface of Hb-loaded MSNs. The morphology and mesostructure of the MSNs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and surface area analysis. The Hb loading capacity (mg/g) in MSNs was studied by thermogravimetric analysis (TGA). UV-Vis absorption spectroscopy revealed that Hb inside MSNs had an identical, but slightly broadened peak in the Soret region compared to free Hb. Furthermore the encapsulated Hb exhibits similar peroxidase-like activity in catalyzing the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The introduction of a supported lipid bilayer (LB) demonstrated the potential to prevent premature Hb release (the burst release decreased from 25.50±0.33% to 6.73±0.83%) and increased the colloidal stability of the Hb-loaded MSNs (hydrodynamic diameter remained ∼250 nm for at least one week). The in vivo systemic circulation and biodistribution of LB-MSNs were studied in optically transparent zebrafish embryos, revealing that LB-MSNs have the potential to act as an erythrocyte mimic in transfusion therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping