PUBLICATION
Identification and characterization of the pumilio-2 expressed in zebrafish embryos and adult tissues
- Authors
- Wang, H.N., Xu, Y., Tao, L.J., Zhou, J., Qiu, M.X., Teng, Y.H., and Deng, F.J.
- ID
- ZDB-PUB-110613-41
- Date
- 2012
- Source
- Molecular biology reports 39(3): 2811-9 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Blotting, Western
- Brain/metabolism
- Cloning, Molecular
- Cluster Analysis
- DNA Primers/genetics
- Embryo, Nonmammalian/metabolism
- Female
- Gene Expression Profiling
- Germ Cells/growth & development*
- Germ Cells/metabolism
- Immunohistochemistry
- In Situ Hybridization
- Male
- Molecular Sequence Data
- Morphogenesis/genetics*
- Ovary/metabolism
- Phylogeny*
- RNA-Binding Proteins/genetics*
- RNA-Binding Proteins/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Testis/metabolism
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish/physiology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 21660475 Full text @ Mol. Biol. Rep.
Citation
Wang, H.N., Xu, Y., Tao, L.J., Zhou, J., Qiu, M.X., Teng, Y.H., and Deng, F.J. (2012) Identification and characterization of the pumilio-2 expressed in zebrafish embryos and adult tissues. Molecular biology reports. 39(3):2811-9.
Abstract
Pumilio proteins regulate the translation of specific proteins required for germ cell development and morphogenesis. In the present study, we have identified the pumilio-2 in zebrafish and analyze its expression in adult tissues and early embryos. Pumilio-2 codes for the full-length Pumilio-2 protein and contains a PUF-domain. When compared to the mammalian and avian Pumilio-2 proteins, zebrafish Pumilio-2 protein was found to contain an additional sequence of 24 amino acid residues within the PUF-domain. Zebrafish pumilio-2 mRNA is expressed in the ovary, testis, liver, kidney and brain but is absent in the heart and muscle as detected by RT-PCR. The results of in situ hybridization indicate that transcripts of pumilio-2 are distributed in all blastomeres from the 1-cell stage to the sphere stage and accumulate in the head and tail during the 60%-epiboly and 3-somite stages. Transcripts were also detected in the brain and neural tube of the 24 h post-fertilization (hpf) embryos. Western blot analyses indicate that the Pumilio-2 protein is strongly expressed in the ovary, testis and brain but not in other tissues. These data suggest that pumilio-2 plays an important role in the development of the zebrafish germ cells and nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping