PUBLICATION
Highly conserved regulatory elements around the SHH gene may contribute to the maintenance of conserved synteny across human chromosome 7q36.3
- Authors
- Goode, D.K., Snell, P., Smith, S.F., Cooke, J.E., and Elgar, G.
- ID
- ZDB-PUB-050610-5
- Date
- 2005
- Source
- Genomics 86(2): 172-181 (Journal)
- Registered Authors
- Cooke, Julie E., Elgar, Greg, Goode, Debbie, Smith, Sarah, Snell, Philip
- Keywords
- Regulatory elements; Enhancers; Fugu; Sonic Hedgehog; Shh; Zebrafish
- MeSH Terms
-
- Animals
- Chromosome Mapping
- Chromosomes, Human, Pair 7*
- Conserved Sequence
- DNA Primers/chemistry
- Enhancer Elements, Genetic
- Genome, Human*
- Green Fluorescent Proteins/metabolism
- Hedgehog Proteins
- Humans
- Models, Genetic
- Molecular Sequence Data
- Regulatory Sequences, Nucleic Acid*
- Sequence Homology, Nucleic Acid
- Synteny*
- Tissue Distribution
- Trans-Activators/genetics*
- Zebrafish
- PubMed
- 15939571 Full text @ Genomics
Citation
Goode, D.K., Snell, P., Smith, S.F., Cooke, J.E., and Elgar, G. (2005) Highly conserved regulatory elements around the SHH gene may contribute to the maintenance of conserved synteny across human chromosome 7q36.3. Genomics. 86(2):172-181.
Abstract
Comparative genomic analysis reveals an exceptionally large section of conserved shared synteny between the human 7q36 chromosomal region and the pufferfish (Fugu rubripes) genome. Remarkably, this conservation extends not only to gene order across 16 genes, but also to the position and orientation of a number of prominent conserved noncoding elements (CNEs). A functional assay using zebrafish has shown that most of the CNEs have reproducible and specific enhancer activity. This enhancer activity is often detected in a subset of tissues which reflect the endogenous expression pattern of a proximal gene, though some CNEs may act over a long range. We propose that the distribution of CNEs, and their probable association with a number of genes throughout the region, imposes a critical constraint on genome architecture, resulting in the maintenance of such a large section of conserved synteny across the vertebrate lineage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping