PUBLICATION
Technology for high-throughput screens: the present and future using zebrafish
- Authors
- Love, D.R., Pichler, F.B., Dodd, A., Copp, B.R., and Greenwood, D.R.
- ID
- ZDB-PUB-041130-8
- Date
- 2004
- Source
- Current opinion in biotechnology 15(6): 564-571 (Review)
- Registered Authors
- Dodd, Andrew, Love, Donald R., Pichler, Franz B.
- Keywords
- none
- MeSH Terms
-
- Animals
- Oligonucleotide Array Sequence Analysis*
- Phenotype
- Polymerase Chain Reaction
- Proteomics*
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/physiology
- PubMed
- 15560983 Full text @ Curr. Opin. Biotechnol.
Citation
Love, D.R., Pichler, F.B., Dodd, A., Copp, B.R., and Greenwood, D.R. (2004) Technology for high-throughput screens: the present and future using zebrafish. Current opinion in biotechnology. 15(6):564-571.
Abstract
The zebrafish is a popular vertebrate model organism with similar organ systems and gene sequences to humans. Zebrafish embryos are optically transparent enabling organ visualisation, which can be complemented with gene expression analysis at the transcript and protein levels. Furthermore, zebrafish can be treated with small molecules and drugs in a microtitre plate format for high-throughput analysis and for the identification and validation of drugs. High-throughput methodologies for use in zebrafish include phenotype-based visualisation, transcript studies using low-density DNA microarrays and proteomic analysis. These technologies offer significant whole-organism biological value in the drug discovery and drug development pipeline.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping