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FIGURE 7 The splice morpholino vwa8-e2i2 induced less severe embryonic abnormalities. Representative micro-images of live zebrafish embryos injected with 5 bp mismatch control morpholino or vwa8-e2i2 (6 ng/embryo). (A) The control embryos were at the 19 somite stage (18 hpf). (B) The vwa8-e2i2 morphants showed developmental delay and were at 50% epiboly stage (6 hpf) simultaneously. (C) Control embryos at 72 hpf showed normal development and straight body, whereas (D) the morphants showed 24 h developmental delay and the curvature at the posterior end. The embryos were overall also smaller than the control.