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FIGURE 6

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ZDB-IMAGE-210923-12
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Figures for Ruggiero et al., 2021
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FIGURE 6

Per2 knockout alters the rhythmic mRNA expression of regulators of key physiological hepatic processes in the adult zebrafish liver. qRT-PCR analysis of expression levels of four CCGs (A)impdh2, (B)hnf1a, (C)cyp1a, and (D)ppargc1b in WT and per2 KO liver. Sets of 4 WT and per2 KO fishes (each set containing two males and two females) were maintained under LD cycle (14 h light-10 h dark) conditions, livers were dissected and pooled for RNA extraction at 6-h intervals during a sampling window of 24 h. Mean mRNA relative expression (n = 2–3) ± SD is plotted on the y-axis; zeitgeber time (ZT) is plotted on the x-axis. Zeitgeber times are indicated for each sample. ZT0 corresponds to lights-on, ZT14 to lights-off. A change in rhythm was observed for impdh2, hnf1a, cyp1a and ppargc1b, as determined by a significant ANOVA Genotype × Time interaction effect for all genes (p < 0.01, see Table 1). Furthermore, hnf1a exhibited a 12 h phase delay, and ppargc1b exhibited a 6 h phase delay. Asterisks represent levels of significance in comparing the two genotypes at each time point individually, corrected by Sidak’s method (***p < 0.001, **p < 0.01, *p < 0.05).

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.