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Fig. 6
Tyr expression is not influenced by alterations in cyp1b1 expression. In situ hybridization demonstrated that at 24 hpf, tyr was expressed in the migrating neural crest and retinal pigment epithelium in uninjected (A) and control-injected (B) embryos. At 48 hpf, tyr expression (A', A'', B', B'') followed the skin pigmentation pattern and was also observed in the cranial neural crest (arrowheads), retinal pigment epithelium, migrating neural crest cells in the ocular fissure (closed arrows), and iris stroma (open arrows). Knockdown of cyp1b1 did not alter tyr expression at 24 hpf, but did decrease tyr expression in the cranial neural crest (arrowhead) and ocular fissure (C, C') at 48 hpf. Overexpression of cyp1b1 did not alter tyr expression (D, D', D'') at 24 and 48 hpf.