PUBLICATION
Infection induced inflammation impairs wound healing through IL-1β signaling
- Authors
- Shen, S., Miskolci, V., Dewey, C.N., Sauer, J.D., Huttenlocher, A.
- ID
- ZDB-PUB-240405-10
- Date
- 2024
- Source
- iScience 27: 109532109532 (Journal)
- Registered Authors
- Huttenlocher, Anna
- Keywords
- Biological sciences, Immune response, Immunology, Microbiology
- Datasets
- GEO:GSE237265
- MeSH Terms
- none
- PubMed
- 38577110 Full text @ iScience
Citation
Shen, S., Miskolci, V., Dewey, C.N., Sauer, J.D., Huttenlocher, A. (2024) Infection induced inflammation impairs wound healing through IL-1β signaling. iScience. 27:109532109532.
Abstract
Wound healing is impaired by infection; however, how microbe-induced inflammation modulates tissue repair remains unclear. We took advantage of the optical transparency of zebrafish and a genetically tractable microbe, Listeria monocytogenes, to probe the role of infection and inflammation in wound healing. Infection with bacteria engineered to activate the inflammasome, Lm-Pyro, induced persistent inflammation and impaired healing despite low bacterial burden. Inflammatory infections induced il1b expression and blocking IL-1R signaling partially rescued wound healing in the presence of persistent infection. We found a critical window of microbial clearance necessary to limit persistent inflammation and enable efficient wound repair. Taken together, our findings suggest that the dynamics of microbe-induced tissue inflammation impacts repair in complex tissue damage independent of bacterial load, with a critical early window for efficient tissue repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping