PUBLICATION
The angiogenesis-modulating effects of coumarin-derivatives
- Authors
- Huang, H.T., Huang, C.Y., Lee, C.J., Sun, B.J., Jhang, Z.W., Wen, C.C., Wang, Y.H., Li, T.S., Chern, C.Y., Chen, Y.H.
- ID
- ZDB-PUB-240222-21
- Date
- 2024
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 278: 109862 (Journal)
- Registered Authors
- Chen, Yau-Hung
- Keywords
- Angiogenesis, Chick, Chick chorioallantoic membrane assay, Coumarin
- MeSH Terms
-
- Angiogenesis*
- Animals
- Biological Assay
- Chickens
- Coumarins/pharmacology
- Mammals
- Zebrafish*
- PubMed
- 38382586 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Huang, H.T., Huang, C.Y., Lee, C.J., Sun, B.J., Jhang, Z.W., Wen, C.C., Wang, Y.H., Li, T.S., Chern, C.Y., Chen, Y.H. (2024) The angiogenesis-modulating effects of coumarin-derivatives. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 278:109862.
Abstract
Coumarin is a natural compound that is rich in plants. Coumarin and its derivates were reported to have many biological activities, such as anti-bacterial, anti-tumor, and anti-coagulation. In this study, we examined the angiogenic modulating activities of six previously synthesized coumarin derivatives (Compound #1-#6) in zebrafish embryos and further confirmed them in a chick model. According to the survival rate in a zebrafish model, Compound #1 (100 %), #2 (82.5-100 %), and #4 (100 %) showed much less toxicity than Compound #3 (19.2-100 %), #5 (0-100 %), and #6 (0-100 %). Using a green blood vessel fluorescent transgenic fish Tg(fli1:egfp) to record the angiogenesis-modulating effects of Compound #1, #2, and #4, we found that Compound #2 had the highest effects in interfering intersegmental vessel growth, subintestinal vein growth, and caudal vein plexus remodeling. Chick chorioallantoic membrane (CAM) assay also showed that Compound #2 exposure led to a reduction of blood vessel growth. Real-time PCR experiments revealed that Compound #2 significantly changed the expression of vascular growth-related genes flt1, cdh5, and nrp1a in zebrafish. Based on our data from zebrafish and chick models, a new coumarin-derivative (Compound #2) possesses anti-angiogenic activity with low toxicity, but further investigation in mammal models is asked to confirm our findings.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping