PUBLICATION

Pou5f1 transcription factor controls zygotic gene activation in vertebrates

Authors
Leichsenring, M., Maes, J., Mössner, R., Driever, W., and Onichtchouk, D.
ID
ZDB-PUB-130903-52
Date
2013
Source
Science (New York, N.Y.)   341(6149): 1005-1009 (Journal)
Registered Authors
Driever, Wolfgang
Keywords
none
Datasets
GEO:GSE41107, GEO:GSE39780
MeSH Terms
  • Animals
  • Binding Sites
  • DNA Polymerase II/metabolism
  • Gene Expression Regulation, Developmental*
  • Octamer Transcription Factor-3/genetics
  • Octamer Transcription Factor-3/metabolism*
  • Pluripotent Stem Cells/cytology
  • Pluripotent Stem Cells/physiology
  • SOXB1 Transcription Factors/metabolism
  • Transcriptional Activation*
  • Xenopus Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Zygote/metabolism*
PubMed
23950494 Full text @ Science
Abstract

The development of multicellular animals is initially controlled by maternal gene products deposited in the oocyte. During the maternal-to-zygotic transition, transcription of zygotic genes commences, and developmental control starts to be regulated by zygotic gene products. In Drosophila, the transcription factor Zelda specifically binds to promoters of the earliest zygotic genes and primes them for activation. It is unknown whether a similar regulation exists in other animals. We found that zebrafish Pou5f1, a homolog of the mammalian pluripotency transcription factor Oct4, occupies SOX-POU binding sites before the onset of zygotic transcription and activates the earliest zygotic genes. Our data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control.

Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping