PUBLICATION
Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy
- Authors
- Shen, W., Meng, A.
- ID
- ZDB-PUB-230615-34
- Date
- 2023
- Source
- STAR protocols 4: 102341102341 (Journal)
- Registered Authors
- Meng, Anming
- Keywords
- Cell Biology, Cell separation/fractionation, Developmental biology
- MeSH Terms
-
- Animals
- Cell Nucleus
- Cytoplasm
- Microscopy, Electron, Scanning
- Nuclear Pore*
- Zebrafish*
- PubMed
- 37314919 Full text @ STAR Protoc
Citation
Shen, W., Meng, A. (2023) Protocol for imaging nuclear pore complexes on isolated nuclei of zebrafish early embryos by field emission scanning electron microscopy. STAR protocols. 4:102341102341.
Abstract
Here, we present a protocol to observe the three-dimensional surface of nuclear pore complexes (NPCs) of vertebrate early embryos by field emission scanning electron microscopy (FESEM). We describe steps from zebrafish early embryo collection and nuclei exposure to FESEM sample preparation and final NPC state analysis. This approach provides an easy way to observe surface morphology of NPCs from the cytoplasmic side. Alternatively, additional purification steps after nuclei exposure supply intact nuclei for further mass spectrometry analysis or other utilization. For complete details on the use and execution of this protocol, please refer to Shen et al.1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping